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Changes in the expression of the T-cell activation markers CD69 and TCRβ in CD4+ and CD8+ subpopulations of CD3+ T cells isolated from spleen and mesenteric lymphatic nodes after their activation via anti-CD3 crosslinking of the T-cell receptor. The data are shown as mean percentages ± SEM. Two-way ANOVA identified the effect of time (p < 10−6), but not the effect of microbiota (p > 0.05) or interaction time*microbiota (p > 0.05).
