Figure 4.

Activation of CCR10 stimulates intracellular Ca²⁺ mobilization. (a) The principle of the NFAT activation assay. Activation of a chemokine receptor leads to phospholipase C (PLC) activation via the transiently expressed Gα₁₅, giving rise to downstream release of Ca²⁺ from the endoplasmic reticulum (ER). Binding of Ca²⁺ to calmodulin (CaM) then enables activation of the protein phosphatase calcineurin (Caln), which catalyzes dephosphorylation of NFAT, enabling its translocation into the nucleus and transcription of the reporter firefly luciferase gene from an NFAT-sensitive reporter construct. (b) Chemokine-induced NFAT activation via CCR10. The presented data are mean ± SEM of at least three independent experiments, each performed in triplicate.