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Dependence of TthPPM activity on various factors: (a) pH and (b) temperature. Reaction conditions: (a) 0.5 mM adenine, 0.5 mM D-ribose 5-phosphate, 0.5 mM MnCl2, 20 mM Tris∙HCl (pH from 7.5 to 9.0), 0.5 µg TthPPM, and 2.7 µg TthPNPII in a 0.25 mL reaction mixture, 80 °C and (b) 0.5 mM adenine, 0.5 mM D-ribose 5-phosphate, 0.5 mM MnCl2, 20 mM Tris∙HCl, pH 8.0, 0.1 µg TthPPM, and 2.7 µg TthPNPII in a 0.25 mL reaction mixture, temperature 35, 50, 70, 80, 90, and 95 °C.
