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. 2021 Apr 16;11(4):586. doi: 10.3390/biom11040586

Figure 2.

Figure 2

Dependence of TthPPM activity on various factors: (a) divalent cations and (b) Mn2+ concentration. Reaction conditions: (a) 0.5 mM adenine; 0.5 mM D-ribose 5-phosphate; 0.5 mM MnCl2, MgCl2, NiCl2, or CaCl2 and without cation; 20 mM Tris∙HCl, pH 8.0; 0.1 µg TthPPM and 2.7 µg TthPNPII in a 0.25 mL reaction mixture, temperature 80 °C and (b) 0.5 mM adenine, 0.5 mM D-ribose 5-phosphate, from 0- to 5 mM MnCl2, 20 mM Tris∙HCl, pH 8.0, 0.1 µg TthPPM, and 2.7 µg TthPNPII in a 0.25 mL reaction mixture, temperature 80 °C.