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Dependence of TthPPM activity on the cofactor concentration. Reaction conditions: 0.5 mM adenine, 0.5 mM D-ribose 5-phosphate, 0.5 mM MnCl2, 20 mM Tris∙HCl, pH 8.0, 0- or 2.5 µM glucose-1,6-bisphosphate, 0.2 µg TthPPM, and 1.4 µg TthPNPII in a 0.25 mL reaction mixture, temperature 80 °C.
