Table 1.
The characteristics of the purified recombinant enzymes.
| Name | Enzyme | Gene | Concentration *, mg/mL | Oligomer form ** | Purity, % *** |
|---|---|---|---|---|---|
| TspRK | Ribokinase Thermus species 2.9 | QT17_05185 | 13.4 | 63 kD adimer |
94.3 |
| TthPPM | Phosphopentamutase Thermus thermophilus HB27 | TT_RS08405 | 10.1 | 43 kD amonomer |
70.5 |
| TthPNPI | Purine nucleoside phosphorylase I Thermus thermophilus HB27 |
TT_RS05405 | 9.8 | 153 kD ahexamer |
96.4 |
| TthPNPII | Purine nucleoside phosphorylase II Thermus thermophilus HB27 |
TT_RS00985 | 10.3 | 179 kD ahexamer |
95.0 |
| EcRK | Ribokinase E. coli K12 | AAA51476.1 | 6.9 | 66 kD adimer |
83.2 |
| EcPPM | Phosphopentamutase E. coli K12 | AAA97279.1 | 11.0 | 44 kD amonomer |
85.4 |
| EcPNP | Purine nucleoside phosphorylase E. coliK12 |
AAA24401.1 | 30.1 | 155 kD ahexamer |
95.8 |
* Analysis by Lowry method. ** Analysis by size exclusion chromatography. *** Analysis by protein electrophoresis in SDS-PAGE.