Table 6.
Conditions for the cascade synthesis of nucleosides (8–10).
| Comp. | D-Pentose, mg (mmol) | Base, mg (mmol) |
Vol., mL |
Enzymes, U | Time, h /Temp, °C |
Isolated Yield, mg (%) |
||
|---|---|---|---|---|---|---|---|---|
| RK | PPM | PNP | ||||||
| Thermus thermophilus | ||||||||
| 8 | Ribose 42.6 (0.284) | 8-Aza-Gua 28 (0.184) |
142 | 11.4 * | 2.8 | 11.4 ** | 24/ 80 |
36.1 (69) |
| Escherichia coli | ||||||||
| 9 | Ribose 42.6 (0.284) | Allop 25 (0.184) |
142 | 5.7 | 2.8 | 11.4 | 24/ 50 |
41.6 (84) |
| 10 | Arabinose 1500 (10) |
(2) 80 (0.25) |
167 | 1670 | 334 | 3000 | 48/ 50 |
43.0 (54 ***) |
| 11 | Arabinose 720 (4.8) |
(6) 36 (0.12) |
80 | 800 | 160 | 1440 | 48/ 50 |
14.7 (41 ***) |
* Ribokinase from Thermus species 2.9 (TspRK, gene QT17_05185) [23] was used. ** Purine nucleoside phosphorylase I (TthPNPI) was used. *** Recalculated for the starting riboside.