Table 4.
Selection of PRRSV forms (full-length sequence and variants) from Porcilis® PRRS vaccine after vaccinations and transmissions during Trial#1 and Trial#2.
| Trial | Inoculated or Contact Pig | Pen | Pig No. | Full-Length Sequence | Variants | % Identity with Variants of Reference (P0) | ||
|---|---|---|---|---|---|---|---|---|
| Long(L) | Short(S) | Shift(F) | ||||||
| #1 | Inoc | 1 | 2012_1 | ND | <1% | >99% | ND | 99.79% |
| Inoc | 1 | 2012_2 | ND | >99% | ND | ND | 99.87% | |
| Inoc | 1 | 2012_3 | ND | ND | 32% | 67% | 99.86% | |
| Cont | 1 | 2012_4 | <1% | >99% | ND | ND | 99.85% | |
| Cont | 1 | 2012_5 | ND | >99% | ND | ND | 99.85% | |
| Cont | 1 | 2012_6 ** | ND | >99% | ND | ND | 99.84% | |
| Inoc | 2 | 2012_7 * | ND | ND | ND | >99% | 99.87% | |
| Inoc | 2 | 2012_8 | ND | >99% | ND | ND | 99.85% | |
| Inoc | 2 | 2012_9 | ND | ND | 50% | 50% | 99.90% | |
| Cont | 2 | 2012_10 | ND | >99% | ND | ND | 99.86% | |
| Cont | 2 | 2012_11 | ND | ND | >99% | ND | 99.85% | |
| Viral form detection frequency in Inoc pigs (≥1%) | 0/6 | 2/6 | 3/6 | 3/6 | ||||
| Viral form detection frequency in Cont pigs (≥1%) | 0/5 | 4/5 | 1/5 | 0/5 | ||||
| Viral form detection frequency in Trial#1 (≥1%) | 0/11 | 6/11 | 4/11 | 3/11 | ||||
| #2 | Inoc | 1 | 2017_12 | ND | >99% | ND | ND | 99.83% |
| Inoc | 1 | 2017_13 | ND | ND | >99% | ND | 99.83% | |
| Inoc | 1 | 2017_14 | ND | <1% | >99% | ND | 99.85% | |
| Cont | 1 | 2017_15 | ND | <1% | >99% | ND | 99.79% | |
| Cont | 1 | 2017_16 | ND | >99% | <1% | ND | 99.82% | |
| Cont | 1 | 2017_17 | ND | <1% | >99% | ND | 99.83% | |
| Inoc | 2 | 2017_18 | ND | ND | >99% | ND | 99.78% | |
| Inoc | 2 | 2017_19 | ND | >99% | ND | ND | 99.85% | |
| Inoc | 2 | 2017_20 | 5% | 44% | 51% | ND | 99.87% | |
| Cont | 2 | 2017_21 | ND | >99% | ND | ND | 99.83% | |
| Cont | 2 | 2017_22 | ND | >99% | ND | ND | 99.83% | |
| Cont | 2 | 2017_23 | ND | >99% | ND | ND | 99.87% | |
| Viral form detection frequency in Inoc pigs (≥1%) | 1/6 | 3/6 | 4/6 | 0/6 | ||||
| Viral form detection frequency in Cont pigs (≥1%) | 0/6 | 4/6 | 2/6 | 0/6 | ||||
| Viral form detection frequency in Trial#2 (≥1%) | 1/12 | 7/12 | 6/12 | 0/12 | ||||
| TOTAL VIRAL FORM DETECTION FREQUENCY (≥1%) | 1/23 | 13/23 | 10/23 | 3/23 | ||||
Inoc, inoculated; Cont, contact; ND abbreviation is used for reads not determined. Identity rates of the major variant present in pigs were determined after an alignment by comparison between sequences from pig samples from Trial#1 and Trial#2 with P0-LONG-DEL, -SHORT-DEL or -SHIFT-DEL parental variants. * Isolate from the pig No. 2012_7 was used as inoculum in Inoc-N-Rev-T#3 inoculated pigs from Trial#3. ** Isolate from the pig No. 2012_6 was used as inoculum in Inoc-Rev-T#3 inoculated pigs from Trial#3.