Table 3.
Gene-specific primers sequence used in the experiment.
| Gene | Accession No. | Primer | Ref. | Annealing |
|---|---|---|---|---|
| β-actin | NM_205518.1 | F: ACCTGAGCGCAAGTACTCTGTCT R: CATCGTACTCCTGCTTGCTGAT |
[25] | 60 |
| Growth Hormone Receptor (GHR) | NM_001001293.1 | F: AACACAGATACCCAACAGCC R: AGAAGTCAGTGTTTGTCAGGG |
[27] | 60 |
| Insulin Like Growth Factor (IGF) | NM_001004384.2 | F: CACCTAAATCTGCACGCT R: CTTGTGGATGGCATGATCT |
[27] | 60 |
| Leptin | AF082500 | F: TCCGCCAAGCAGAGGGGT R: CCAGGACGCCATCCAGGCTCTCTGGC |
[28] | 58 |
| Cholecystokinin (CCK) | NM_001001741.1 | F: CAGCAGAGCCTGACAGAACC R: AGAGAACCTCCCAGTGGAACC |
[29] | 58 |