Table 6.

miRNA and mRNA sequence parameters.

miRNA or mRNA	miRNA or mRNA Accession Number (miRbase, Unigene)	Nucleotide Sequence of PCR Primer, 5’-3’	PCR Primers Annealing Temperature, °C
hsa-miR-1-3p	MIMAT0000416	TGGAATGTAAAGAAGTATGTAT	52.7
hsa-miR-199a-3p	MIMAT0000232	ACAGTAGTCTGCACATTGGTTA	57.6
hsa-miR-199b-3p	MIMAT0004563	ACAGTAGTCTGCACATTGGTTA	57.6
hsa-miR-185-3p	MIMAT0004611	AGGGGCTGGCTTTCCTCTGGTC	48.9
hsa-miR-140-3p	MIMAT0004597	TACCACAGGGTAGAACCACGG	48.9
hsa-miR-30c-5p	MIMAT0000244	TGTAAACATCCTACACTCTCAGC	57.6
hsa-miR-654-3p	MIMAT0004814	TATGTCTGCTGACCATCACCTT	52.7
hsa-miR-15b-5p	MIMAT0000417	TAGCAGCACATCATGGTTTACA	57.6
hsa-miR-128-3p	MIMAT0000424	TCACAGTGAACCGGTCTCTTT	59
hsa-miR-28-3p	MIMAT0004502	CACTAGATTGTGAGCTCCTGGA	54
hsa-miR-30d-5p	MIMAT0000245	TGTAAACATCCCCGACTGGAAG	54
SNORD68	NR_002450	Hs_SNORD68_11 miScript Primer Assay, Qiagen	55
ANXA2 F	NM_004039.3	CAGCATTTGGGGACGCTCTCA	48.9
ANXA2 R		AATGGTGACCTCATCCACACC
TAGLN2 F	NM_003564.3	CCCTCACTGTGCTGCTCTTT	52.7
TAGLN2 R		GCCATCCTTGAGCCAGTTCT
YWHAZ F	NM_003406.4	ACGACGTCCCTCAAACCTTG	52.7
YWHAZ R		TGACCTACGGGCTCCTACAA
FN1 F	XM_017003692.1	TGGTGCCATGACAATGGTGT	48.9
FN1 R		CGGGAATCTTCTCTGTCAGCC
FLNA F	NM_001456.4	AGTGTCAATCGGAGGTCACG	61.6
FLNA R		CTGGTCACATCCAGCCCATT
DSTN F	NM_001011546.2	AGTGTTGGGCCAGGCTTTAG	57.6
DSTN R		TGCATCCTTGGAGCTTGCAT
FBN1 F	NM_000138.5	ACATCTCCGCGTGTATCGAC	57.6
FBN1 R		CACAGGTCCCACTTAGGCAG
VIM F	NM_003380.5	GGACCAGCTAACCAACGACA	46.2
VIM R		AAGGTCAAGACGTGCCAGAG
MYH11 F	NM_002474.3	AGCGGTACTTCTCAGGGCTA	52.7
MYH11 R		AGATGGGCCTTGCGTGATAC
FGA F	NM_000508.5	GCAAAGATTCAGACTGGCCC	61.6
FGA R		CTACTGCATGACCCTCGACA
MYL6 tr.v.1 F	NM_021019.5	ACTTCACCGAAGACCAGACC	57.6
MYL6 tr.v.1 R		TATGCCTCACAAACGCTTCATAG
MYL6 tr.v.2 F	NM_079423.4	ACTTCACCGAAGACCAGACC	52.7
MYL6 tr.v.2 R		GCGGACGAGCTCTTCATAGT
GAPDH F	NM_002046	ACCACAGTCCATGCCATCAC	60
GAPDH R		TCCACCACCCTGTTGCTGTA
ACTB F	NM_001101	GGACTTCGAGCAAGAGATGG	60
ACTB R		AGCACTGTGTTGGCGTACAG