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. Author manuscript; available in PMC: 2021 Apr 26.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2020 Feb 27;40(4):929–942. doi: 10.1161/ATVBAHA.119.313698

Figure 1. Lymph collection and lymph platelet extracellular vesicles (PEV) quantification.

Figure 1.

A, Lymph is collected at the thoracic duct, between the transverse lumbar artery and the diaphragm in a collection tube coated with EDTA (0.1 M). B, Representative picture of translucent collected lymph. C and D, Red blood cells (RBCs; C) and platelet (D) counts in blood, spun blood (plasma), and unprocessed lymph (n=3). E, Efficient flow cytometry quantification of PEV in lymph was verified by spiking and serial-dilutions of in vitro-generated PEV in either lymph or PBS. Nondiluted (ND), n=3. F, Sensitivity to Triton 0.05% of the PEV quantified by flow cytometry (n=4). G, PEV were quantified in platelet-free plasma and in lymph in C57BL/6 wild-type mice (n=5).