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. 2021 Apr 19;22(8):4220. doi: 10.3390/ijms22084220

Figure 2.

Figure 2

Genistein induces the mRNA expression of transcription factor EB (TFEB)-target genes and increases TFEB levels in NPC1 patient fibroblasts. (A) The graph shows qPCR analysis of mRNA levels of different TFEB target genes in GM05659 wild type (WT) fibroblast and GM03123 NPC1 patient fibroblasts after 24 h treatment with 150 µM of genistein. The bar graphs represent the relative fold induction of these genes normalized with GAPDH mRNA levels. Representative immunoblot (B) and quantification (C) of endogenous TFEB total levels in lysates from WT fibroblast and NPC1 patients fibroblast obtained after 1, 3, 6, 12, and 24 h treatment with 150 µM of genistein. GAPDH was used as a loading control. Data were presented as mean ± SEM of three independent samples (each with triplicates), * p ≤ 0.05, analyzed by ANOVA with Tukey posttest, n = 3 for each experiment (in triplicates for genes).