Skip to main content
. Author manuscript; available in PMC: 2022 Mar 1.
Published in final edited form as: FEBS Lett. 2021 Feb 9;595(5):577–594. doi: 10.1002/1873-3468.14033

Fig. 10.

Fig. 10.

Fluorescently activated cell sorting (FACS) analysis measuring d2EGFP expression in clone 2B5 cells following 16hrs of TCR activation using anti-CD3 and anti-CD28 mAbs. Clone 2B5 cells harbor single latently infected proviruses with mNF-κB LTR which blocks binding of both NF-κB and NFAT to the HIV promoter. Clone 2B5 cells were activated through the TCR using anti-CD3 and anti-CD28 mAbs for 16 hrs and analyzed for d2EGFP expression. The mutated NF-κB binding sites are required for the activation of HIV transcription by NF-κB and NFAT.