Fig. 7.

Fluorescently activated cell sorting (FACS) analysis to determine d2EGEP expression. Prior to 16hrs of activation, Clone 2D10 cells were either untreated or pretreated for 1hr with PD, CsA, or PD/CsA drug inhibitors to specifically block induction of AP-1, NFAT, or both AP-1/NFAT transcription factors, respectively. (A) Drug controls (B) FACS analysis of untreated and drug-treated samples following TNF-α stimulation and (C) FACS analysis of untreated and drug-treated samples following TCR activation using anti-CD3 and anti-CD28 mAbs. Note that the results presented here represent experiments that were performed three times and each time samples were analyzed in duplicates. Error bars represent the mean ± SD of three independent and separate experiments. The P value of statistical significance was set as P < 0.05 (*).