Figure 3.

TR triggered autophagy in human leukemia Jurkat cells. (A) Whole cell lysates from Jurkat cells treated with 10 µM TR for indicated time periods were subjected to Western blot analysis for autophagy-related proteins expression levels. Densitometry measurements, normalized to β-actin are indicated below the corresponding blot. The data are represented as the mean of at least three independent experiments. (B) Jurkat cells were incubated with 10 µM TR for 24 h, followed by stained simultaneously with 50 nM LysoTracker Green and 5 µM Hoechst33342 in culture media for 30 min and analyzed by fluorescence microscopy (magnification 1000×, scale bars 10 µM).