Skip to main content
. 2021 Apr 20;11(4):365. doi: 10.3390/life11040365

Figure 5.

Figure 5

TR promotes apoptosis and autophagy through inhibition of PI3K/AKT/mTOR and Ras/Raf/MEK/ERK signaling and activation of AMPK in human leukemia Jurkat cells. Whole cell lysates from Jurkat cells treated with 10 µM TR for indicated time periods were subjected to Western blot analysis and investigated for (A) PI3K, p-PI3K (Tyr199/458), AKT, p-AKT (Ser473), mTOR, p-mTOR (Ser2228) proteins expression levels, (B) Ras, B-Raf, p-B-Raf (Ser445), MEK, p-MEK (Ser217/221), ERK, p-ERK (Thr202/Tyr204) proteins expression levels and (C) AMPK, p-AMPK (Thr172) proteins expression levels. Quantification results of p-PI3K/PI3K, p-AKT/AKT, p-mTOR/mTOR, Ras/β-actin, p-B-Raf/Raf, p-MEK/MEK and p-ERK/ERK are indicated below the corresponding blot. The data are represented as the mean of at least three independent experiments.