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Peripheral blood mononuclear cell (PBMC) intracellular cytokine staining (ICS). PBMC ICS antigen-specific responses in two latent Mycobacterium tuberculosis (M.tb)-infected UK adults vaccinated with 1 × 107 pfu aerosol-inhaled MVA85A. Percentages of CD4+ T cells producing IFN-γ, TNF-α, IL-2, IL-17, and CD8+ T cells producing IFN-γ and TNF-α in response to (A) Ag85A, (B) PPD, (C) ESAT-6, and (D) CFP-10. Individual values shown for each volunteer.
