Table 2.

The chemical composition of obtained extract (E1 and E2) and CPC fraction.

Parameter	1 Step Extraction (Crude Extract E1)	2 Step Extraction (Extract E2, CO2 b or CO2/EtOHc)	CPC Fractionation
α-acids, % m/m	42.48 ± 0.68	n.a. a	n.a. a
β-acids, % m/m	19.07 ± 0.30	n.a. a	n.a. a
xanthohumol, wt%,	n.a. a	4.80 ± 0.58 c	81.70 ± 0.66
	n.a. a	6.50 ± 0.43 b [1]	n.a a
TPC, mgGAE/g	n.a. a	52.19 ± 1.11 c	n.a. a
	n.a. a	48.71 ± 0.76 b	n.a. a
extraction yield, wt%	11.40	4.64 b	n.a. a
		10.76 c	n.a. a

^a n.a.—not applicable; ^b CO₂—extraction with pure carbon dioxide; ^c CO₂/EtOH—extraction with carbon dioxide modified with EtOH (96%) (99:1, v/w).