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. 2021 Apr 18;22(8):4198. doi: 10.3390/ijms22084198

Figure 5.

Figure 5

Effects of exercise training in HDF-fed male APOB-100 transgenic mice. At the end of the experiment, (A) body weight was measured and serum samples were collected to determine the concentration of (B) serum glucose, leptin, TG, TNFα, and resistin. Lipid accumulation in the liver and brown adipose tissue was also analysed. (C) Liver and (E) BAT sections were stained with hematoxylin and eosin. Scale bar: 200 μm. Weight of the (D) liver and (F) BAT were measured and the number, size, and area of lipid droplets in the (D) liver and in the (F) BAT were quantified using ImageJ software. Blue striped columns represent the differences in the given value in regularly trained APOB-100/HFD male mice, expressed as the percentage of the corresponding value for sedentary APOB-100/HFD males. n = 12 mice per group for body, liver, and BAT weight and serum glucose measurements, n = 10 mice per group for serum leptin, TNFα, and resistin concentration measurements, n = 6 mice per group for the quantification of lipid accumulation. Values are presented as mean ± SEM; * denotes p < 0.05; ** denotes p < 0.01; *** denotes p < 0.001. WT/ND, wild-type mice on normal diet; APOB/HFD, APOB-100 transgenic mice on high-fat diet; BAT, brown adipose tissue; l.d., lipid droplet.