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. 2021 Apr 20;22(8):4263. doi: 10.3390/ijms22084263

Figure 6.

Figure 6

PRI-724 significantly activated caspase 3/7 and induced cell death. (A) Luminometric measurement of viability and caspase 3/7 activity in GCT cell lines treated with PRI-724 showed increased activity of this caspase after cisplatin PRI-724 treatment, where NTERA-2 CisR cells showed the highest activity. Values were expressed as percentage of the untreated cells (control) ± SD. (B) Annexin V assay revealed significant increase in population of NCCIT CisR cells in early apoptosis, increase in late apoptosis/necrosis in TCam-2 cells and necrotic population of NTERA-2 CisR cells after PRI-724 treatment. Values were expressed as relative positivity of the untreated cells (control) ± SD. 7-AAD = necrosis, Annexin V + 7-AAD = late apoptosis/necrosis, Annexin V = early apoptosis. * p < 0.05, ** p < 0.01.