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. 2021 Apr 20;22(8):4263. doi: 10.3390/ijms22084263

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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PRI-724 significantly inhibited migratory capacity of NTERA-2 CisR cells. (A) PRI-724 significantly decreased the migration of NTERA-2 CisR cells out of the 3D spheroid. Migratory capacity of other GCT cell lines was not affected by PRI-724 treatment. Images were taken 3 days post transfer from non-adherent to adherent conditions. (B) Migration of NTERA-2 CisR cells pretreated (for 72 h before plating) or treated (for 36 h) with PRI-724 was significantly decreased compared to untreated NTERA-2 CisR cells in a wound healing assay. Confluent monolayers of NTERA-2 CisR cells were wounded and cell migration was observed by live-cell imaging for 36 h. * p < 0.05.