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. 2021 Apr 20;22(8):4263. doi: 10.3390/ijms22084263

Figure 9.

Figure 9

PRI-724 treatment did not affect protein levels of β-catenin and cyclin D1 in GCT cell lines. (A) qPCR analysis revealed significant decrease in expression of CTNNB1 in NTERA-2 CisR and TCam-2 CisR cells and increased CTNNB1 expression in JEG-3 CisR cells treated with PRI-724 inhibitor. (B) Decreased CCND1 expression was confirmed in NTERA-2 CisR cells after PRI-724 treatment. Significant upregulation was detected in PRI-724 treated NCCIT CisR and TCam-2 CisR cells. (C) Western blot and densitometric analysis confirmed that PRI-724 treatment did not change the protein levels of β-catenin and cyclin D1 in GCT cell lines. 1. TCam-2 CisR; 2. TCam-2 CisR + PRI-724; 3. NTERA-2 CisR; 4. NTERA-2 CisR + PRI-724; 5. NCCIT CisR; 6. NCCIT CisR + PRI-724; 7. JEG-3 CisR; 8. JEG-3 CisR + PRI-724. β-actin was used as an internal loading control. * p < 0.05, *** p < 0.001.