Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Apr 16;13(8):1927. doi: 10.3390/cancers13081927

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Effect of ADAM12 expression on cell proliferation of human CRC cells. (A) Expression of ADAM12 protein via gene transfection. Western blotting results revealed that ADAM12 protein was overexpressed and knocked down using ADAM12-pcDNA6-myc construct and ADAM12 siRNA, respectively. (B) Effect of ADAM12 expression on proliferation was determined using WST-1 assay in human CRC cells (* p < 0.05). (C) Plate colony formation assay confirmed single-cell proliferative capability due to ADAM12 expression (* p < 0.05). (D) Effect of ADAM12 expression on BrdU incorporation of proliferating human CRC cells (* p < 0.05). ADAM12, A disintegrin and metalloprotease 12; CRC, colorectal cancer; EV, EmptyV, empty-pcDNA6-myc vector; AV, ADAM12-pcDNA6-myc construct; SS, scramble siRNA; AS, ADAM12 siRNA; BrdU, bromodeoxyuridine.