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. 2021 Apr 16;10(4):919. doi: 10.3390/cells10040919

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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miRNA-145 overexpression induces chronic activation of intracellular signalling. (a) Cells were transfected with premiRNA-145 or premiRNA -ve for 6 h, placed into 0.4% FCS for 96 h and protein lysates prepared. These were immunoblotted for (b) p38, (c) ERK, (d) Akt (all expression and phosphorylation), (e) IκB, (f) p21 expression. Phosphorylation of proteins was measured against expression of the same protein, whereas changes in expression only were measured against α-tubulin as a loading control (n = 6–9).