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. 2021 Apr 16;26(8):2320. doi: 10.3390/molecules26082320

Figure 6.

Figure 6

Curcumin inhibits LPA initiated MCP-1 expression via blocking ROCK signalling. (A) VSMCs were pre-incubated with 10 μM curcumin for 30 min before treatment of LPA (10 μM) for 15 min. Blots were probed with primary antibodies specific to phospho-Ezrin (Thr567)/Radixin (Thr567)/Moesin (Thr558) (1:2000) and secondary HRP conjugated rabbit IgG antibody (1:2000). Blots are representative of three independent experiments. (B) VSMCs were pre-incubated with 10 μM Y27632 for 30 min before treatment with LPA (10 µM) for 2 h. Total RNA was harvested and assessed by qRT-PCR. 18S was used as a house keeping gene. Results are expressed as mean ± SEM from three independent experiments. Statistical significance was determined by one-way ANOVA, followed by least significant difference post-hoc analysis. * p < 0.05 and ** p < 0.01 versus basal; ## p < 0.01 versus LPA only treated sample.