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. 2021 Apr 16;22(8):4122. doi: 10.3390/ijms22084122

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Effects of systemically subchronic (for 7 days: light blue columns) and chronic (for 14 days: blue columns) administrations of guanfacine on expression of α2A adrenoceptor in the plasma membrane of LC (Panel (A)), OFC (Panel (B)) and VTA (Panel (C)), using capillary immunoblotting system. Ordinate: mean ± SD (n = 6) of the relative protein level of α2A adrenoceptor (ADRA2A) in the plasma membrane fraction. Effects of guanfacine on α2A adrenoceptor expression were analysed by one-way ANOVA with Tukey’s post hoc test (* p < 0.05, ** p < 0.01 vs. Control). The pseudo-gel images, using capillary immunoblotting were represented in the lower panels.