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. 2021 Apr 20;22(8):4253. doi: 10.3390/ijms22084253

Figure 9.

Figure 9

Putative upstream regulators (pURs) of MgSO4 effects on the HI transcription response. (A) Activation of Ifng appeared to be a significant activated pUR (p = 9.01 × 10−13, z = 4.472) 12 h after HI, putatively activating 42 genes also associated with inflammation and immunity CPs (top panel). In MgHI, it did not remain a significant pUR (p > 1 × 10−2, z = 3.111) although it was associated with the activation of 31 genes (bottom panel). Only 7 genes were common to the two conditions. (B) The inhibition of IL4 by MgSO4 in naïve mice identified it as a pUR (p = z = −3.561) associated with the repression of 24 genes, of which several were also recorded as part of CPs (top panel). IL4 activation in the HI response makes it a pUR of inflammation activating 17 genes involved in this function (lower panel). IPA® did not consider IL4 a pUR in MgHI groups. (C) Activation of Ppara (p = 4.02 × 10−4, z = 2.302) in HI 12 h after insult showed it to be a pUR in the HI response in Ctrl mice, as well as the possible upstream negative regulator of cholesterol biosynthesis repressor (highlighted in blue, top panel). MgSO4 pretreatment reversed activation of Ppara with pUR inhibitory activity on function, at least at a short time post insult (bottom panel). *; multiple identifiers (probes) in data list.