Figure 5.

Expression of INS proteins in life cycle stages of Cryptosporidium parvum. The expression of CpINS-4 (A) and CpINS-6 (B) on C. parvum oocyst (top panel), sporozoites (second panel), merozoites (third panel) and intracellular developmental stages in HCT-8 cell cultures at 24 h (fourth panel) and 48 h (bottom panel) was assessed by immunofluorescence using the polyclonal antibodies generated. The images were taken under differential interference contrast (DIC), with nuclei being counter-stained with 4′, 6-diamidino-2-phenylindole (DAPI), parasites stained by immunofluorescence assay with anti-INS antibodies, and superimposition of the three images (Merged). Scale-bars: 2 µm for all figures.