Figure 1.

AAV.coRPGR plasmid used in this study. (A) Circular map for the AAV.coRPGR expression plasmid used to quantify the AAV8.RPGR titer by Quantitative real-time PCR (qPCR). This plasmid was previously used in a pre-clinical study [20] and contains the rhodopsin kinase promoter (GRK1) that will drive the expression of a codon optimized version of RPGR^ORF15 followed by a polyadenylation signal. This transgene cassette is flanked by inverted terminal repeats (ITRs) from AAV2 required for replication and packaging. The restriction enzyme HindIII was used to linearize the plasmid. (B) Diagram showing the linearized plasmid following digestion with HindIII restriction enzyme. The green bars indicate the target sequences where the primers and probe bind, i.e., the GRK1 promoter and the bGH polyA (pA) sequence. Abbreviations: CDS; coding sequence, ITR; inverted terminal repeat, AmpR; ampicillin resistance gene.