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Primers and probes designed to quantify AAV.RPGR titre.
| Target | Label | Sequence (5′→3′) |
|---|---|---|
| GRK1 promoter | Forward primer | TCTCTTAAGGTAGCCCCGG |
| Reverse primer | ATCCGATTAGATCATTCTGCCC | |
| Taqman Probe (FAM) | CCTCACTTTTCCCCTGAGAAGGACA | |
| bGH polyA | Forward primer | CTCGACTGTGCCTTCTAGTTG |
| Reverse primer | ACCTACTCAGACAATGCGATG | |
| Taqman Probe (FAM) | TGCCACTCCCACTGTCCTTTCC |
