Table 2.

PCR efficiency and performance on undigested and digested plasmid DNA.

	GRK1 Promoter		bGH polyA
	Supercoiled	HindIII-Linearized	Supercoiled	HindIII-Linearized
Ct range	12.66–28.87	10.72–28.91	13.21–28.91	10.81–28.53
R square	0.9953	0.9993	0.9923	0.9989
Slope	−3.153	−3.607	−3.080	−3.487
Intercept	39.13	41.31	38.81	40.42
Efficiency	107.6%	89.4%	110.2%	93.5%