. 2021 Apr 20;10(4):343. doi: 10.3390/biology10040343

Table 2.

N-glycan number, structure, accurate mass, mass used for quantification, corresponding adduct, and the heavy reference number used for LC-MS/MS and MALDI-TOF/MS quantification methods.

Nr.	Proposed Structure, Theoretical Mass, and Composition	Mass Used for Quantitation	Adduct	Heavy Reference		Structure Confirmed by MS/MS Data ^a
				LC-MS/MS	MALDI
6	HexNac2Hex5	1579.7826	[M + Na]⁺	52	6	✓
8	HexNAc3Hex4	1620.8090	[M + Na]⁺	19	-	*
10	HexNAc2Hex6	903.4358	[M + 2Na]²⁺	52	6	✓✓
14	HexNAc4Hex4	1865.9355	[M + Na]⁺	19	19	✓✓
17	HexNAc2Hex7	1005.4857 *	[M + 2Na]²⁺	52	6	✓✓
22	HexNAc5Hex4	1067.0255	[M + 2Na]²⁺	19	-	*
23	HexNAc6Hex3	1087.5388 *	[M + 2Na]²⁺	13	-	✓
25	HexNAc2Hex8	1107.5356	[M + 2Na]²⁺	52	6	✓✓
26	HexNAc4Hex4NeuAc1	1125.0492	[M + 2Na]²⁺	26	35	✓✓
28	HexNAc5Hex4Fuc1	1154.0701	[M + 2Na]²⁺	19	19	✓
31	HexNAc3Hex5Fuc1NeuAc1	1191.5805	[M + 2Na]²⁺	27	-	✓✓
33	HexNAc2Hex9	1209.5855	[M + 2Na]²⁺	52	6	✓✓
36	HexNAc5Hex4NeuAc1	1247.6123	[M + 2Na]²⁺	52	-	✓✓
40	HexNAc3Hex6Fuc1NeuAc1	1293.6304 *	[M + 2Na]²⁺	27	-	✓✓
41	HexNAc7Hex3Fuc1	1297.1465	[M + 2Na]²⁺	75	-	*
42	HexNAc2Hex10	1311.6353	[M + 2Na]²⁺	52	-	✓
43	HexNAc7Hex4	1312.1518	[M + 2Na]²⁺	72	-	✓
44	HexNAc4Hex5Fuc1NeuAc1	1314.1437	[M + 2Na]²⁺	44	58	✓✓
49	HexNAc5Hex5NeuAc1	907.4379	[M + 3Na]³⁺	35		✓
51	HexNAc6Hex6	936.7887	[M + 3Na]³⁺	52	35	✓
52	HexNAc4Hex5NeuAc2	1407.6859	[M + 2Na]²⁺	52	52	✓✓
55	HexNAc5Hex6NeuAc1	975.4711	[M + 3Na]³⁺	72	35	✓✓
56	HexNAc6Hex5Fuc2	984.8149	[M + 3Na]³⁺	27	-	✓
57	HexNAc6Hex6Fuc1	994.8184	[M + 3Na]³⁺	27	-	✓
58	HexNAc4Hex5Fuc1NeuAc2	1004.1501	[M + 3Na]³⁺	58	58	✓✓
61	HexNAc5Hex6Fuc1NeuAc1	1033.5009 *	[M + 3Na]³⁺	27	-	✓
64	HexNAc5Hex5Fuc1NeuAc2	1617.2937	[M + 2Na]²⁺	27	64	✓
66	HexNAc6Hex6Fuc1NeuAc1	1115.2096	[M + 3Na]³⁺	27	-	✓
67	HexNAc6Hex7Fuc2	1669.8275 *	[M + 2Na]²⁺	27	-	✓
79	HexNAc6Hex7NeuAc3	1030.2440	[M + 4Na]⁴⁺	80	52	✓
85	HexNAc7Hex8NeuAc4	1232.8439 *	[M + 4Na]⁴⁺	80	-	*
86	HexNAc7Hex8Fuc1NeuAc4	1276.3663 *	[M + 4Na]⁴⁺	80	-	✓
87	HexNAc4Hex3Fuc2	1016.5017	[M + 2Na]²⁺	13	-	✓✓

^a one check indicates that permethylated structure was confirmed, double check indicates that both structures (permethylated and perdeuterated) were confirmed, while asterisk indicates that only perdeuterated structure was confirmed. Legend: Inline graphic N-acetyl glucosamine (GlcNac), ● Mannose (Man), ● Galactose (Gal), ◄ Fucose (Fuc), Sialic acid (NeuAc).