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Antibody titers evaluated by ELISA in bovine sera immunized on day 0 with vaccine A and day 30 with vaccine D. Group 1 = vaccine A; group 4 = vaccine D; group 7 = vaccine A + D; and Control group = not vaccinated. Mean titers ± standard errors (SEM) are shown. Letters (a, b and c) highlight significant differences (p < 0.05) between groups. A: Total IgG antibodies. B: IgG1 subclass antibodies. C: IgG2 subclass antibodies.
