Fig. 1.

PEDV infection reduces endogenous expression of TRIM21. Vero and LLC-PK1 cells were mock infected or infected with PEDV at an MOI of 1, and the cells were collected and processed at the indicated time points. (A) The mRNA level of TRIM21 in Vero cells was measured by real-time PCR. (B) The protein levels of TRIM21, N, and β-actin in Vero cells were measured by western blotting using the indicated antibodies. β-actin was used as the sample loading control. (C) The mRNA level of TRIM21 in LLC-PK1 cells was measured by real-time PCR. (D) TRIM21, N, and β-actin protein levels in LLC-PK1 cells were measured by western blot using the indicated antibodies. β-actin was used as the sample loading control. Data are the mean ± SD of triplicate samples. *, p < 0.05; **, p < 0.01; ***, p < 0.001 (two-tailed Student’s t-test)