Table 1.
Purpose | Primer | Sequence (5′-3′) |
---|---|---|
Real-time PCR | PEDV N forward | GAGGGTGTTTTCTGGGTTG |
PEDV N reverse | CGTGAAGTAGGAGGTGTGTTAG | |
SUS TRIM21 forward | CCAGACTCCCCTCTACCCT | |
SUS TRIM21 reverse | TTCCACCGTCATTGAAACC | |
MACACA TRIM21 forward | CCTTCGTGGAGCCTGTGAGC | |
MACACA TRIM21 reverse | GGCGGAGATTCCTGAGCAGA | |
β-actin forward | TCCCTGGAGAAGAGCTACGA | |
β-actin reverse | AGCACTGTGTTGGCGTACAG | |
siRNA assay | si-mTRIM21 sense | GGAAUGCAUCUCUCAGGUUTT |
si-mTRIM21antisense | AACCUGAGAGAUGCAUUCCTT | |
NC sense | UUCUCCGAACGUGUCACGUTT | |
NC antisense | ACGUGACACGUUCGGAGAATT |