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. 2021 Apr 20;13(4):712. doi: 10.3390/v13040712

Figure 1.

Figure 1

(E)-Guggulsterone inhibits DENV replication in a dose-dependent manner. (a) (E)-guggulsterone chemical structure. (E)-guggulsterone efficiently inhibited DENV (b) protein synthesis and (c) RNA replication in a dose-dependent manner. DENV-infected Huh-7 cells were treated with 0.1% of DMSO (dose 0, consider as the negative control), 2.5, 10, or 20 μM (E)-guggulsterone for 3 days. The DENV protein and RNA levels were analyzed by Western blotting and RT-qPCR, respectively. The percent inhibition was relative to the cells treated with 0.1% DMSO (corrected to “0”) with DENV infection defined as 100%. (d) Cell viability of (E)-guggulsterone. Huh-7 cells were treated with the indicated concentrations of (E)-guggulsterone for 3 days, and the cell viability was assessed using an MTS assay. Error bars denote the mean ± SD of three independent experiments (n = 3). * p < 0.05; ** p < 0.01.