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. 2021 Apr 26;21:233. doi: 10.1186/s12935-021-01841-y

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — LncRNA VIM-AS1 is upregulated in bladder cancer and is related tobladder cancer metastasis. a, b The expression of lncRNA VIM-AS1 was determined in 35 paired bladder cancer and noncancerous tissue samples, and analyzed in 15 nonmetastatic and 20 metastatic bladder cancer tissues. c Vimentin expression was determined in 35 paired bladder cancer and noncancerous tissue samples by RT-PCR. d The correlation of VIM-AS1 and Vimentin expression in tissue samples was analyzed using Pearson’s correlation analysis. e, f The expression of Ki67 and Vimentin in tissue samples were determined by IHC staining (×200). g The expression of VIM-AS1 and Vimentin was determined in a normal cell line, SV-HUC-1, three high-metastatic bladder cancer cell line (TCCSUP, HT-1376, and T24 and a low-metastatic bladder cancer cell line RT4 using real-time PCR. h, i T24 and RT24 cells were stimulated with 0, 2, 5 and 10 ng/ml TGFβ1 and examined for the expression of VIM-AS1 and Vimentin using real-time PCR. *P < 0.05, **P < 0.01