Fig. 4.

Dynamic effects of VIM-AS1 and miR-655 on bladder cancer metastasis. a, d T24 cells were co-transfected with si-VIM-AS1 and miR-655 inhibitor; RT24 cells were co-transfected with VIM-AS1 and miR-655 mimics; the protein levels of Vimentin, E-cadherin, and Snail were determined by immunoblotting. b, e The invasive ability of T24 and RT24 cells was determined by Transwell assay. Scale bar = 100 µm. c, f The migratory ability of T24 and RT24 cells was determined by wound healing assay. Scale bar = 100 µm. *P < 0.05, **P < 0.01 compared to inhibitor NC + si-NC or mimics NC + vector group. ^##P < 0.01 compared to miR-655 inhibitor + si-NC or miR-655 mimics + vector group