Fig. 6.

Dynamic effects of VIM-AS1 and ZEB1 on bladder cancer metastasis. a–c T24 cells were cotransfected with si-VIM-AS1 and ZEB1 vector and the expression levels of VIM-AS1 (a), ZEB1 (b) and miR-655 (c) were detected by real-time PCR analysis. d, j The protein levels of Vimentin, E-cadherin, Snail and ZEB1 were determined by immunoblotting in T24 and RT24 cells. e, k The invasive ability of T24 and RT24 cells was determined by Transwell assay. Scale bar = 100 µm. f, l The migratory ability of T24 and RT24 cells was determined by wound healing assay. Scale bar = 100 µm. g–i RT24 cells were cotransfected with VIM-AS1 vector and si-ZEB1 and the expression levels of VIM-AS1 (g), ZEB1 (h) and miR-655 (i) were detected by real-time PCR assay. **P < 0.01 compared to si-NC + vector group. ^##P < 0.01 compared to si-NC + ZEB1 or vector + si-ZEB1 group