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. 2021 Apr 26;21:233. doi: 10.1186/s12935-021-01841-y

Table 1.

The primer sequence

Name Forward Reverse

RT-PCR

VIM-AS1

CTCAGACCTGTAGCATCAGCATCAC TTCATTTCCTCATAAGCGAACACTC

RT-PCR

ZEB1

AGCAGTGAAAGAGAAGGGAATGC GGTCCTCTTCAGGTGCCTCAG

RT-PCR

Vimentin

AGTCCACTGAGTACCGGAGAC CATTTCACGCATCTGGCGTTC

RT-PCR

GAPDH

ACAGCCTCAAGATCATCAGC GGTCATGAGTCCTTCCACGAT

RT-PCR

miR-655-3p

RT:GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACAAAGAG

F: GCCGCATAATACATGGTTAAC

CAGTGCGTGTCGTGGA

RT-PCR

U6

CTCGCTTCGGCAGCACA AACGCTTCACGAATTTGCGT
NC mimics UUCUCCGAACGUGUCACGUTT ACGUGACACGUUCGGAGAATT
NC inhibitor CAGUACUUUUGUGUAGUACAA
MiR-655 mimics AUAAUACAUGGUUAACCUCUUU AGAGGUUAACCAUGUAUUAUUU
MiR-655 inhibitor AAAGAGGUUAACCAUGUAUUAU
Si-VIM-AS1 1# GGAUGUGUAAGAGAAUUUATT UAAAUUCUCUUACACAUCCTT
Si-VIM-AS1 2# CCAUGUGUGCGAUUCACAATT UUGUGAAUCGCACACAUGGTT
Si-ZEB1 CCUAGUCAGCCACCUUUAATT UUAAAGGUGGCUGACUAGGTT
Si-NC UUCUCCGAACGUGUCACGUTT ACGUGACACGUUCGGAGAATT
Wt-VIM-AS1 aattctaggcgatcgctcgagAGATTTTAAAATAGAGCATGCTTAATATACTT attttattgcggccagcggccgcCACTAGTACACCCCCGACGTG
Mut-VIM-AS1 AGTAtgcgctGTATTGGCTGTCCCACATTTTGA GCCAATACagcgcaTACTACTAAGTAATAAATCTATGTCATCCAAAGG
Wt-ZEB1 3′UTR aattctaggcgatcgctcgagATTTGGCTCATAACTGTTTCCAAA attttattgcggccagcggccgcTTTCATACTAAAATATATTATTGTATTAATACAAACTAC
Mut-ZEB1 3′UTR AgcgcgcaTACCCTTCTTACTGACATATGTACTTTTAGTT AGAAGGGTAtgcgcgcTGCCAGGTTTGAAGACATACAGTAC

Lv-VIM-AS1

overexpression

ctaccggactcagatctcgagTTCTCCCGGAGGCGCATG gtaccgtcgactgcagaattcTTTTTTTTTAGCATATCCAAAAATGG

Lv-miR-655-3p

overexpression

ctaccggactcagatctcgagATATTTTTAATGGAACCTGCCTTAGA gtaccgtcgactgcagaattcACAGCAAACACAGCAAAGCAAC
ZEB1 overexpression ctaccggactcagatctcgagATGAAAGTTACAAATTATAATAC gtaccgtcgactgcagaattcTTAGGCTTCATTTGTCTTTTC