Table 4.

Study 2: Efficacy of ProHeart 12 compared to Heartgard Plus or Interceptor Plus against the JYD-34 heartworm strain: Day 165 inoculation, adult heartworm AG and MF results

Treatment	Dosage	Days of treatment	Number of infected dogsa, b	No. dogs positive for adult heartworm AGc		No. dogs positive for MF
				Day 331 (day 166 PI)	Day 360 (day 195 PI)	Day 331 (day 166 PI)	Day 360 (day 195 PI)
Negative control	NA	0	6 of 6	6 of 6d	6 of 6e	0 of 6	5 of 6
ProHeart 12	0.5 mg/kg moxidectinf	0	4 of 6	0 of 6g	1 of 6h	0 of 6	0 of 6
Heartgard Plus	Min. of 6 µg/kg ivermectini	0, 30, 60, 90, 120, 150, 180, 210, 240, 270, 300, 330	6 of 6	6 of 6j	6 of 6	0 of 6	5 of 6
Interceptor Plus	Min. of 0.5 mg/kg milbemycin oximek	0, 30, 60, 90, 120, 150, 180, 210, 240, 270, 300, 330	6 of 6	6 of 6l	6 of 6m	0 of 6	5 of 6

^aEach dog was inoculated with 50 D. immitis third-stage larvae (JYD-34 strain) on day 165

^bAll dogs were necropsied for recovery of adult D. immitis on day 360 (195 days PI; 30 days after the last treatment with Heartgard Plus and Interceptor Plus)

^cDiroCHEK

^d Two of six dogs positive only after heat treatment; positive on both DiroCHEK and Snap4DX

^eOne of six dogs positive only after heat treatment; positive on both DiroCHEK and Snap4DX

^f ProHeart 12 (moxidectin extended-release injectable suspension) administered according to commercial label directions which resulted in a point administration of 0.5 mg/kg of moxidectin to each dog

^gAll six of the dogs remained Ag-negative after samples were heat treated

^hOne dog with a single male worm was slightly positive only after heat treatment; positive on both DiroCHEK and Snap4DX

ⁱHeartgard Plus (ivermectin + pyrantel) administered according to commercial label directions which resulted in administration of 6.5 to 11.5 µg/kg of ivermectin

^jThree of six dogs positive only after heat treatment; positive on both DiroCHEK and Snap4DX

^kInterceptor Plus (milbemycin oxime + praziquantel) administered according to commercial label directions which resulted in administration of 0.51 to 1.1 mg/kg milbemycin oxime

^lFive of six dogs positive only after heat treatment; positive on both DiroCHEK and Snap4DX

^mOne of six dogs positive only after heat treatment; positive on both DiroCHEK and Snap4DX