Fig. 4.

The VEN/8-Cl-Ado combination downregulates proteins involved in fatty acid metabolism and inhibits fatty acid uptake in AML cells. a Western blotting with antibodies against proteins with decreased mRNA expression, as assessed by GSEA for fatty acid metabolism of the MV4-11 cell line treated with VEN/8-Cl-Ado for 24 h. Primary AML blasts (pool of cells from 5 patients) and MV4-11 and KG-1a cells were treated for 24 h with 500 nM 8-Cl-Ado and 10 nM VEN, or control, prior to cell lysis and Western blotting with antibodies against CD36, TP53INP2, ACAT2, IDI1 and actin (loading control). b Fatty acid uptake assay was performed in primary AML blasts and KG-1a and MV4-11 cells treated for 24 h as described in a followed by fatty acid uptake assay