Table 1.
Germline usage and mutation frequencies of the isolated NTD-specific mAbs
| mAb | IGHV | IGHD | IGHJ | IGLV | IGLJ | CDR3 length |
SHMa |
||
|---|---|---|---|---|---|---|---|---|---|
| VH | VL | VH | VL | ||||||
| BLN1 | IGHV1-24 | IGHD1-20 | IGHJ6 | IGKV2-24 | IGKJ2 | 21 | 9 | 6 | 11 |
| BLN2 | IGHV1-24 | IGHD3-10 | IGHJ6 | IGLV2-14 | IGLJ2 | 21 | 10 | 3 | 10 |
| BLN3 | IGHV1-24 | IGHD1-20 | IGHJ6 | IGLV2-14 | IGLJ2 | 21 | 12 | 11 | 12 |
| BLN4 | IGHV4-30 | IGHD3-22 | IGHJ3 | IGLV1-44 | IGLJ1 | 21 | 11 | 5 | 6 |
| BLN5 | IGHV1-24 | IGHD1-20 | IGHJ6 | IGLV2-23 | IGLJ3 | 21 | 11 | 12 | 14 |
| BLN6 | IGHV4-61 | IGHD3-22 | IGHJ3 | IGLV2-8 | IGLJ1 | 21 | 10 | 4 | 12 |
| BLN7 | IGHV1-24 | IGHD6-19 | IGHJ5 | IGLV2-14 | IGLJ1 | 14 | 10 | 4 | 9 |
| BLN8 | IGHV3-30 | IGHD6-19 | IGHJ5 | IGLV3-1 | IGLJ2 | 10 | 9 | 4 | 3 |
| BLN9 | IGHV1-24 | IGHD6-19 | IGHJ5 | IGLV2-14 | IGLJ2 | 14 | 12 | 5 | 13 |
| BLN10 | IGHV1-24 | IGHD6-13 | IGHJ5 | IGLV3-19 | IGLJ2 | 16 | 10 | 4 | 22 |
| BLN12 | IGHV1-24 | IGHD1-20 | IGHJ6 | IGKV2-24 | IGKJ4 | 21 | 9 | 8 | 3 |
| BLN14 | IGHV3-30 | IGHD3-3 | IGHJ6 | IGKV3-20 | IGKJ5 | 16 | 8 | 4 | 1 |
DNA sequences of the antibodies were analyzed by IgBlast, for determining the germline allele usage and CDR3 loop length (Ye et al., 2013). See Figure S2 for full amino acid sequences.
a
No. of somatic hypermutations (SHM) in the DNA level, relative to the respective V germline gene.