Table 2.
Binding kinetic parameters of the selected NTD-specific mAbs, determined by BLI
| mAb | Kon (1/Ms) | Koff (1/s) | KD (nM) |
|---|---|---|---|
| BLN1 | 2.47 × 105 | 1.57 × 10−4 | 0.7 |
| BLN2 | 1.81 × 105 | 2.53 × 10−3 | 14 |
| BLN3 | 3.14 × 105 | 5.75 × 10−4 | 1.8 |
| BLN4 | 1.19 × 105 | 4.12 × 10−3 | 34 |
| BLN5 | 2.63 × 105 | 3.72 × 10−4 | 1.4 |
| BLN6 | 1.94 × 105 | 3.07 × 10−3 | 15.8 |
| BLN7 | 2.86 × 105 | 7.77 × 10−4 | 2.7 |
| BLN8 | 1.35 × 105 | 5.83 × 10−4 | 4.3 |
| BLN9 | 2.35 × 105 | 2.81 × 10−3 | 12 |
| BLN10 | 2.11 × 105 | 9.08 × 10−4 | 4.3 |
| BLN12 | 3.63 × 105 | 3.31 × 10−4 | 0.9 |
| BLN14 | 9.31 × 104 | 1.18 × 10−3 | 12.7 |
Each tested mAb was biotinylated, immobilized on a streptavidin sensor, and interacted with increasing amounts of NTD. Binding kinetics curves were fitted using the 1:1 binding model (see Figure S4).