| AI | Artificial insemination |
| ANOVA | Analysis of variance |
| CASA | Computer assisted sperm analysis |
| CFU | Colony-forming unit |
| DE | Digestible energy |
| EB | Tris-citric acid-glucose supplemented with EDTA and bestatin |
| EDTA | Ethylenediaminetetraacetic acid |
| HOST | Hypo-osmotic swelling test |
| LSM | Least square means |
| NAR | Normal apical ridge |
| PI | Iodide propidium |
| QEB | Tris-citric acid-glucose supplemented with EDTA, bestatin and nanoparticles of chitosan-alginate |
| SEM | Standard error of the means |
| TCG | Tris-citric acid-glucose |
| TCG-AB | Tris-citric acid-glucose without antibiotics |
| TCG+AB | Tris-citric acid-glucose plus antibiotics |
| VRBD | Violet Red Bile Dextrose |