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. 2021 Apr 20;13(8):1987. doi: 10.3390/cancers13081987

Figure 4.

Figure 4

bFGF and VEGF seem to be the key growth factors down-regulating decorin expression in human stromal fibroblasts. (A) Human breast stromal fibroblasts were serum starved for 24 h, pre-incubated with 20 μΜ of SU5402 for 1 h and then treated with PDGF (10 ng/mL), bFGF (10 ng/mL), EGF (100 ng/mL), IGF-I (100 ng/mL) or TGF-β (5 ng/mL) for another 24 h before RNA extraction, first-strand cDNA synthesis and real-time PCR analysis. (B) Young and ionizing radiation-induced senescent human breast stromal fibroblasts were incubated for 24 h with the designated growth factor receptor inhibitors (SU5402 for FGFR and VEGFR (20 μΜ); SU5416 for VEGFR (1 μΜ); AZD4547 for FGFR (21 nΜ)). RNA was extracted and decorin (dcn) expression was estimated by RT-qPCR with glyceraldehyde-3-phosphate dehydrogenase (GAPDH) serving as the reference gene. Relative mRNA levels compared to the respective untreated control are presented as means ± standard deviations of three experiments. Statistically significant differences in comparison to the respective control without the inhibitor are marked by asterisks (*) (Student’s t test, p < 0.05). IR: ionizing radiation.