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Position of the forward and reverse Ceph18S primers on the cephalopod 18S rRNA gene. Nucleotide diversity (π) was calculated with a sliding-window analysis (window size = 99 bp, step size = 10 bp) over the SILVA alignment (after removal of large indels 0–572, 2004–2411, 6255–6529) and shown based on the reference sequence of L. formosana (accession code: AY557478).
