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Isoflurane and SIRT1 mRNA expression and activity. Wild-type mice were exposed to air or 2% isoflurane for 1 h and cortical tissue was harvested immediately and at 6, 12, and 36 h after isoflurane exposure were subjected to RT-PCR and ELISA. Data indicate mean ± SEM. (A) **** p < 0.0001 vs. control (no isoflurane) by ANOVA, ns—nonsignificant. (B) ns—nonsignificant, p > 0.05 vs. control (no isoflurane) by ANOVA. (n = 5).
