Skip to main content
. 2021 Apr 20;22(8):4287. doi: 10.3390/ijms22084287

Figure 1.

Figure 1

Ultrastructural analysis of non-small cell lung cancer (NSCLC) A549 cell spheroids. Representative images of NSCLC A549 cells showing cell surface architecture in a 2D adhesive culture dish (left panel) and a 3D poly-HEMA-coated dish (right panel). The images were taken after 3 days of culturing. The cells in the 2D monolayer culture were treated with 1 µM jasplakinolide for 1 h. Then, the cells were cultured on 2D tissue culture dishes or poly-HEMA-coated dishes. Upper panel: The representative transmission electron microscopy images of NSCLC A549 cells grown in 2D (left) and 3D (right) cultures. Middle panel: The representative scanning electron microscopy images of NSCLC A549 cells grown in 2D (left) and 3D (right) cultures. Lower panel: The phase-contrast micrographs showing the morphologies of human NSCLC A549 cells grown in 2D (left) and 3D (right) cultures. White arrowhead indicates tight junction; Black arrowhead indicates protrusion. Note the adherens junctions and protrusions. Original magnification: ×1000. Scale bar: 200 μm.