Figure 2.

Representative live-cell three-dimensional holotomography of NSCLC A549 cells. The cells were exposed to 30% deionized water and 5% sucrose to give an osmotic shock to induce hypotonic stress and hypertonic stress, respectively. Images were taken for 10 h upon seeding the cells. The cells in the 3D poly-HEMA-coated nonadhesive dishes (lower panel) show exploratory protrusions compared to the cells in the 2D normal adhesive culture dish (upper panel). Additionally, see Supplementary Movies 1 and 2.